Lecture
High-throughput analysis on open droplet arrays
- 09.04.2024 at 16:30 - 17:00
- ICM Saal 4b
- Language: English
- Type: Lecture
Lecture description
Droplet microfluidics is a powerful method for high throughput screening, e.g. for screening reaction conditions, synthesizing particles, or single-cell analysis and other bioanalytical tasks. We employ droplet-based methods an open platform, which is conceptionally similar to a multi-well plate, however, has a massively increased density of wells and hence, throughput. Thousands of aqueous nL-droplets deposited on a custom-made glass slide that have a defined hydrophilic-hydrophobic surface pattern. The droplets are covered by fluorinated oil and remain stable for several days. Compounds and fluids can be added by further spotting runs at any time. Moreover, our automated deposition system allows for the creation of chemical gradients along the surface and therefore, fine-tuned concentration-depending screening applications. In addition, we can employ optical microscopy for droplet assessment as well as MALDI-MS imaging for analysis of droplet composition.
In this presentation, the use and versatility of the method for various applications will be discussed. After validation of the platform, we first confirmed the use of the platform for protein analysis and determination of posttranslational modifications. Next, we adapted the platform for cell analysis. For example, the biosynthesis of an enzyme could be monitored by both fluorogenic assay as well as label-free by mass spectrometry. Further recent advancements of the platform include the analysis of supernatant by droplet splitting, the production of chemical gradients and the use of hydrogels for embedding cells.
In this presentation, the use and versatility of the method for various applications will be discussed. After validation of the platform, we first confirmed the use of the platform for protein analysis and determination of posttranslational modifications. Next, we adapted the platform for cell analysis. For example, the biosynthesis of an enzyme could be monitored by both fluorogenic assay as well as label-free by mass spectrometry. Further recent advancements of the platform include the analysis of supernatant by droplet splitting, the production of chemical gradients and the use of hydrogels for embedding cells.
All lectures within this session
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- 15:30 - 16:00Enabling data-science in the laboratory with open formats and infrastructure
- 16:00 - 16:30On the road of digital transformation: Building the fully automated and digitalized lab of the future
- 16:30 - 17:00High-throughput analysis on open droplet arrays