Lecture
Immunochemical Sensing of Markers in the Water Cycle
- 10.04.2024 at 10:30 - 11:00
- ICM Saal 3
- Language: English
- Type: Lecture
Lecture description
In recent decades, many organic micropollutants, often referred to as emerging contam-inants, have been found in waters from the whole water cycle, including wastewater, surface water, groundwater, and drinking water. Trace analytical methods are needed for monitoring their distribution and concentration trends. Moreover, to cover the whole water cycle and allow for frequent sampling, methods should be fast, cost-effective, ma-trix-tolerant, and portable, at best, true sensors. Immunoanalytical methods, employing antibodies for the sensing step, can provide the necessary selectivity and sensitivity. Since they employ one antibody per compound, most formats are single analyte methods or, at best, allow for oligoplexing. Therefore, to harness their advantages, indicators of contamination should be chosen where no legally binding lists exist. These markers should be able to talk about input of micropollutants into the water cycle, their (partial) elimination in wastewater treatment steps, the input of residues into receiving waters and their potential appearance in groundwater and tap water.
The talk will present as base application the ELISA (enzyme-linked immunosorbent as-say) based on microtiter plates as the method of choice for analysing a large number of samples [1]. ELISAs are available for monitoring anthropogenic markers such as the an-tiepileptic drug carbamazepine, the analgesic diclofenac, the antihistamine cetirizine, the steroid hormone estrone, the antimicrobial sulfamethoxazole, the psychoactive sub-stances caffeine and cocaine, the priority pollutant bisphenol A, and isolithocholic acid, a bile acid. Simpler formats, such as mix-and-read assays, e.g., the fluorescence polari-zation immunoassay (FPIA) [2] or the lateral flow immunoassay (LFIA) [3], are more suit-able for on-site screening and monitoring. The latter is based on dipsticks or small cas-settes, with which users have become familiar during the COVID-19 pandemic through rapid antigen tests. The suitability of multi-analyte formats such as immunomicroarrays depends on the choice of a signal generation system that offers small uncertainties and good reproducibility. Bead-based (“suspension”) arrays read out in flow cytometers are a powerful platform for multiplexed assays [4]. Electrochemical formats running on port-able microfluidic devices offer additional advantages as no light source is required. They are particularly promising for stand-alone analysers and biosensors [5]. Speed, low cost and on-site capabilities of immunochemical sensing permits much more data to be col-lected on markers, enabling for the quantification of inputs and outputs, thus allowing for a differentiated picture of the contamination of the water cycle to be drawn up.
Literature:
[1] Bahlmann, Carvalho, Weller, Panne, Schneider, Chemosphere, 2012, 89, 1278.
[2] Oberleitner, Dahmen-Levison, Garbe, Schneider, J. Environ. Manage., 2017, 193, 92.
[3] Raysyan, Schneider, Biosensors, 2021, 11, 231.
[4] Carl, Sarma, Gregório, Hoff-mann, Lehmann, Rurack, Schneider, Anal. Chem., 2019, 91, 12988.
[5] Abdelshafi, Bell, Rurack, Schneider, Drug Test Anal., 2019, 11, 492
The talk will present as base application the ELISA (enzyme-linked immunosorbent as-say) based on microtiter plates as the method of choice for analysing a large number of samples [1]. ELISAs are available for monitoring anthropogenic markers such as the an-tiepileptic drug carbamazepine, the analgesic diclofenac, the antihistamine cetirizine, the steroid hormone estrone, the antimicrobial sulfamethoxazole, the psychoactive sub-stances caffeine and cocaine, the priority pollutant bisphenol A, and isolithocholic acid, a bile acid. Simpler formats, such as mix-and-read assays, e.g., the fluorescence polari-zation immunoassay (FPIA) [2] or the lateral flow immunoassay (LFIA) [3], are more suit-able for on-site screening and monitoring. The latter is based on dipsticks or small cas-settes, with which users have become familiar during the COVID-19 pandemic through rapid antigen tests. The suitability of multi-analyte formats such as immunomicroarrays depends on the choice of a signal generation system that offers small uncertainties and good reproducibility. Bead-based (“suspension”) arrays read out in flow cytometers are a powerful platform for multiplexed assays [4]. Electrochemical formats running on port-able microfluidic devices offer additional advantages as no light source is required. They are particularly promising for stand-alone analysers and biosensors [5]. Speed, low cost and on-site capabilities of immunochemical sensing permits much more data to be col-lected on markers, enabling for the quantification of inputs and outputs, thus allowing for a differentiated picture of the contamination of the water cycle to be drawn up.
Literature:
[1] Bahlmann, Carvalho, Weller, Panne, Schneider, Chemosphere, 2012, 89, 1278.
[2] Oberleitner, Dahmen-Levison, Garbe, Schneider, J. Environ. Manage., 2017, 193, 92.
[3] Raysyan, Schneider, Biosensors, 2021, 11, 231.
[4] Carl, Sarma, Gregório, Hoff-mann, Lehmann, Rurack, Schneider, Anal. Chem., 2019, 91, 12988.
[5] Abdelshafi, Bell, Rurack, Schneider, Drug Test Anal., 2019, 11, 492
All lectures within this session
- 09:30 - 10:00The miniRUEDI is a portable mass spectrometer for efficient on-site quantification of dissolved gases in aquatic systems
- 10:00 - 10:30Real Time Measurement of Microbial Water Quality
- 10:30 - 11:00Immunochemical Sensing of Markers in the Water Cycle
- 11:00 - 11:30Sensors in Environmental Practice