Lecture
Less Work, Better Peaks - solution to solvent effects challenge in LC & LC/MS analysis
- 09.04.2024 at 10:30 - 11:00
- ICM Saal 5
- Language: English
- Type: Lecture
Lecture description
Feed injection is a solution to the challenge of strong solvent effects in LC and LC/MS analysis. This technique mediates the strong solvent effect by infusing the sample into the mobile phase stream. This allows you to achieve perfect peak shapes and easy peak integration without the need for additional sample preparation. This presentation will give you a comprehensive introduction to Feed injection technology, complemented by application examples from food safety and PFAS analysis.
Feed Injection is used for the multiresidue LC/MS analysis of polar pesticides dissolved in strong eluting organic solvent. The sample is infused at various feed speeds, mitigating solvent effects and trapping and enriching the polar compounds on the column. It eliminates breakthrough and provides better peak shapes, which improves detection, quantification, and fully automated peak integration. A typical multiresidue method where this technique can be applied is the analysis of pesticides extracted from fruits and vegetables using pure acetonitrile in QuEChERS sample preparation. [1]
Feed Injection is also used for the analysis of per- and polyfluorinated alkyl substances (PFAS). The optimized Feed Speed is used for the sample injection to trap and enrich the compounds on the column. This avoided peak broadening and breakthrough of the early-eluting polar PFAS compounds. The less polar PFAS compounds can be enriched for more sensitive quantification. [2]
Literature:
[1] Edgar Naegele, Improved Peak Shape and Lower LOQs in Pesticide Analysis. Agilent Technologies application note, publication number 5994-6125EN, 2023.
[2] Matthias Kamuf, Edgar Naegele, More Sensitive Quantification of PFAS by LC/MS with the Agilent 1260 Infinity II Hybrid Multisampler. Agilent Technologies application note, publication number 5994-6994EN, 2024.
Feed Injection is used for the multiresidue LC/MS analysis of polar pesticides dissolved in strong eluting organic solvent. The sample is infused at various feed speeds, mitigating solvent effects and trapping and enriching the polar compounds on the column. It eliminates breakthrough and provides better peak shapes, which improves detection, quantification, and fully automated peak integration. A typical multiresidue method where this technique can be applied is the analysis of pesticides extracted from fruits and vegetables using pure acetonitrile in QuEChERS sample preparation. [1]
Feed Injection is also used for the analysis of per- and polyfluorinated alkyl substances (PFAS). The optimized Feed Speed is used for the sample injection to trap and enrich the compounds on the column. This avoided peak broadening and breakthrough of the early-eluting polar PFAS compounds. The less polar PFAS compounds can be enriched for more sensitive quantification. [2]
Literature:
[1] Edgar Naegele, Improved Peak Shape and Lower LOQs in Pesticide Analysis. Agilent Technologies application note, publication number 5994-6125EN, 2023.
[2] Matthias Kamuf, Edgar Naegele, More Sensitive Quantification of PFAS by LC/MS with the Agilent 1260 Infinity II Hybrid Multisampler. Agilent Technologies application note, publication number 5994-6994EN, 2024.
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